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This is especially accurate for immunohistochemistry through which 10% NBF is shown to get a bad fixative (Fig. 2). Some problems with using 10% NBF being a fixative are corrected via the introduction of other experimental approaches for example antigen retrieval (Shi et al. 2001) and the use of compact amplicons in RT-Q-PCR (Steg et al. 2006, 2007

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